Methods Immunohistochemical method and Bismark brown staining were used respectively for the observation of proliferating cell nuclear antigen ( PCNA) and mast cell ( MC). 方法用免疫组织化学方法和俾士麦棕法分别检测100例乳腺癌组织中增殖细胞核抗原(PCNA)和肥大细胞(MC)。
Methods: Bismark brown staining method was used for the observation of MC, and monoclonal antibody CD43 was used for marking the endothelial surface. 方法:俾士麦棕法显示胃癌MC,单克隆抗体CD34标记血管内皮。